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PerCP-Cyanine5.5 - anti mouse CD62L - Clone MEL-14
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PerCP-Cyanine5.5 - anti mouse CD62L - Clone MEL-14
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PerCP-Cyanine5.5 - anti Mouse CD62L - MEL-14

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105,00 € tax excl.

290,00 € tax excl.

Product Description

  • MEL-14
  • Rat IgG2a
  • Flow Cytometry
  • PerCP-Cyanine5.5
  • 0.2 mg/ml
  • C3H/eb mouse B lymph oma 38C-13
  • Mouse
  • The reagent is provided in aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3)
  • RUO
  • CD62L, clone MEL-14, is a mAb intended for RUO in the identification of cells that express CD62L. This reagent is effective for direct IF staining of mouse sample for FCM analysis using ≤1 μg/106 cells.
  • Liquid
  • Store in the dark at 2-8 °C
CD62L (L-selectin), binds a 95 kDa (on neutrophils) or 74 kDa (on lymph ocytes) receptor with lectin-like and Epidermal Growth Factor-like domains. In the mouse, L-selectin is detected on most thymocytes, with the highest levels of expression on an immunocompetent subset and a population of dividing progenitor cells, and on peripheral leukocytes, including subsets of B and T lymph ocytes, neutrophils, monocytes, and eosinophils. This member of the selectin adhesion molecule family appears to be required for lymph ocyte homing to peripheral lymph nodes and to contribute to neutrophil emigration at inflammatory sites. L-selectin is rapidly shed from lymph ocytes and neutrophils upon cellular activation; metalloproteinases may mediate the release of CD62L ectodomains from the cell surface. The level of CD62L expression, along with other markers, distinguishes naive, effector, and memory T cells. L-selectin binds to sialytaed oligosaccharide determinants on high endothelial venules (HEV) in peripheral lymph nodes. In vitro studies have demonstrated that CD34, GlyCAM-1, and MAdCAM-1, all recognized by mAb MECA-79 (anti-mouse PNAd Carbohydrate Epitope, may be ligands for CD62L. MEL-14 mAb blocks in vitro binding of lymph ocytes to peripheral lymph node HEV and inhibits in vivo lymph ocyte extravasation into peripheral lymph nodes and late stages of leukocyte rolling.
  • Sell; L-selectin; LECAM-1; LAM-1; Lnhr; Ly-22; Ly-m22; Lyam-1
  • 20343
  • 95 kD (neutrophils) or 74 kD (lymphocytes)
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