Large description: Apoptosis is characterized by a variety of morphological features. One of the earliest indications of apoptosis is the translocations of the membrane phospholipid phosphatidylserine (PS) from the inner to the outer leaflet of the plasma membrane. Once exposed to the extracellular environment, s on PS become available for Annexin V, a 35-36 kDa, Ca 2+-dependent, phospholipid binding protein with a high affinity for PS. The translocations of PS precedes other apoptotic processes such as loss of plasma membrane integrity, DNA fragmentation, and chromatin condensation. As such, Annexin V can be conjugated to biotin or to a fluorochrome, and used for the easy, flow cytometric identification of cells in the early stages of apoptosis.
Aplication Reference:
Vagace JM et al. Etiopathological mechanisms and clinical characteristics of hyperhemolysis syndrome in Spanish patients with thalassemia. 2016 Annals of Hematology
Other names: Annexin A5
GeneID: 308
Molecular weight: 35,9 kDa
