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> 98% pure by SDS-PAGE ; migrates as five distinct bands ( two doublets ) when run undernaturing polyacrylamide SDS-urea gels

0 . 01ng / ml( Lowest concentration of toxin at which a positive response ( clustered growth pattern ) is obtained is > or=0.01)

0.1 mg/ml

Reconstitution of one vial with 0.5 ml distilled water will give a solution containing 50ug of pertussis toxin , 0 . 05M sodium phosphate and 0 . 5M sodium chloride at PH 7.2

Store at 2-8°C when lyophilised or resuspended Use within 1 month